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RSC Advances-13 April 2021

發布日期:2021-09-27 16:52:51 【關閉】
摘要:Standards used in this study, including naringenin, naringenin chalcone and pinocembrin chalcone were obtained from Chengdu DeSiTe Biological Technology.

Two putative parallel pathways for naringenin biosynthesis in Epimedium wushanense
期刊名:RSC Advances
文獻編號:DOI: 10.1039/d1ra00866h
文獻地址:https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA00866H
發表日期:13 April 2021

Abstract

Flavonoids that exhibit various biological activities such as antioxidant, antitumor, antiviral, antibacterial and anti-inflammatory properties are found in a wide range of medicinal plants. Among the flavonoid-producing plants identified so far, the genus Epimedium is recognised as a group of prolific prenyl-flavonoid glycoside producers with high economic value in the global dietary supplement market. To date, the biosynthetic genes for prenyl-flavonoid glycosides still remain elusive in Epimedium. Here, we identified five genes in Epimedium wushanense responsible for the biosynthesis of naringenin, the common precursor for flavonoid natural products. We successfully set up the biosynthetic pathway of naringenin using L-tyrosine as the precursor through enzymatic assays of these genes' encoding products, including phenylalanine ammonia-lyase (EwPAL), 4-coumarate-CoA ligase (Ew4CL1), chalcone synthase (EwCHS1), chalcone isomerase (EwCHI1) and CHI-like protein (EwCHIL3). Intriguingly, in vitrocharacterisation of the above catalytic enzymes' substrate specificity indicated a route parallel to naringenin biosynthesis, which starts from L-phenylalanine and ends in pinocembrin. The fact that there is no pinocembrin or pinocembrin-derived flavonoid accumulated in E. wushanense prompted us to propose that pinocembrin is likely converted into naringenin in vivo, constituting two parallel biosynthetic pathways for naringenin. Therefore, our study provides a basis for the full elucidation of the biosynthetic logic of prenyl-flavonoid glycoside in Epimedium, paving the way for future metabolite engineering and molecular breeding of E. wushanense to acquire a higher titre of desired, bioactive flavonoid compounds.
 

Standards used in this study, including naringenin, naringenin chalcone and pinocembrin chalcone were obtained from Chengdu DeSiTe Biological Technology.


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